[Other] IN CHINEESE OR ENHLISH : Optimization of Fermentation Conditions in a Shaker for the Recombinant Manganese Superoxide Dismutase from Engineering E.coli Copy

Fermentation media(carbon source,nitrogen source,inorganic salt)and cultivation conditions(inoculum quantity,lactose concentration,time,temperature,shaker speed,pH value etc.) by single factor researches were preliminarily optimized in a shaker for the recombinant manganese superoxide dismutase from engineering E.coli.The results showed that the optimized culture media for the engineering E.coli were 1.5 % trytone,1.5 % yeast extract,1.0 % NaCl,0.4 % KH2PO4,0.8 % K2HPO4,1.5 % glycerine,0.2 % NH4Cl and 5 mmol/L MnCl2.The optimized lactose induction conditions were growth for 5 h after inoculation at 5 % inoculum quantity,and then adding 0.25 % lactose to induce for 6 h.The enzyme specific activity was the highest when the fermentation temperature was 37 ℃,the shaker speed was 180 r/min,and the original medium pH value was 7.0.Under all of these optimized conditions,SOD from the engineering E.coli exhibited the enzymatic activity of 1 969.9 U/mL and the specific activity of 1 081.8 U/mg.