Harmonizing amplification and cleavage kinetics through crRNA scaffold mutation enables robust one-pot CRISPR–Cas12 diagnostics

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journal：Proceedings of the National Academy of Sciences

Authors：Qingyang Jiang; Ruiquan Xu; Zhixin Lin; Chenyu Sun; Feidi Ye; Yanyan Ye; Duoming Lin; Zhen Li; Jingwen Mai; Zhihong Lin; Jiuxin Qu; Xiaoyan Deng; Chenchen Ge; Dou Wang

Published date：2026-9-15

DOI：10.1073/pnas.2613134123

PDF link：https://pnas.org/doi/pdf/10.1073/pnas.2613134123

Article link：https://doi.org/10.1073/pnas.2613134123

Article Source：National Academy of Sciences。

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