Super-resolution microscopy demystified

Post time Yesterday 23:48

DOI: 10.1038/s41556-018-0251-8
Abstract
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.

Lothar Schermelleh 1, Alexia Ferrand 2, Thomas Huser 3, Christian Eggeling 4 5, Markus Sauer 6, Oliver Biehlmaier 2, Gregor P C Drummen 7Nat Cell Bio 2019 Jan;21(1):72-84 Epub 2019 Jan 2.

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